Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells
|ournal||FEBS LETT 590, 2671-2680 (2016)|
|Authors||Tsuchiya M (1), Ogawa H (1, 2), Koujin T (2), Kobayashi S (2), Mori C (2), Hiraoka Y (1, 2), Haraguchi T (1, 2).
1.Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
2.Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
|Title||Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells|
|Laboratory||Nuclear Dynamics Group (Prof. Hiraoka)|
|Abstract||Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.|